1W3G
Hemolytic lectin from the mushroom Laetiporus sulphureus complexed with two N-acetyllactosamine molecules.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2004-06-10 |
| Detector | ENRAF-NONIUS |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 101.720, 101.720, 192.910 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.680 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w3a |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.780 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.150 | 0.450 |
| Number of reflections | 17326 | |
| <I/σ(I)> | 15.6 | 2.2 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 15.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | pH 4.50 |
