1W3F
Crystal structure of the hemolytic lectin from the mushroom Laetiporus sulphureus complexed with N-acetyllactosamine in the gamma motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF NONIUS |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2004-05-25 |
| Detector | Nonius Kappa CCD |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 101.760, 101.760, 193.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.850 - 2.580 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w3a |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALE |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.720 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.090 | 0.440 |
| Number of reflections | 19024 | |
| <I/σ(I)> | 7.6 | 1.4 |
| Completeness [%] | 98.1 | 98.1 |
| Redundancy | 9 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | pH 4.50 |
