1W3B
The superhelical TPR domain of O-linked GlcNAc transferase reveals structural similarities to importin alpha.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Collection date | 2003-11-07 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.320, 75.510, 77.670 |
| Unit cell angles | 105.08, 105.14, 110.28 |
Refinement procedure
| Resolution | 200.000 - 2.850 |
| R-factor | 0.2594 |
| Rwork | 0.259 |
| R-free | 0.29740 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.254 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.050 | 0.470 |
| Number of reflections | 27841 | |
| <I/σ(I)> | 21 | 3.5 |
| Completeness [%] | 97.3 | 96.8 |
| Redundancy | 5.8 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M HEPES-NA/HCL PH 7.5 0.2M CACL2 36% PEG 400 |
