1W32
The 3-dimensional structure of a thermostable mutant of a xylanase (Xyn10A) from Cellvibrio japonicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 120 |
| Collection date | 2001-12-10 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 95.343, 95.343, 150.273 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.650 - 1.200 |
| R-factor | 0.122 |
| Rwork | 0.121 |
| R-free | 0.14400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1clx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.406 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.080 | 0.340 |
| Number of reflections | 213551 | |
| <I/σ(I)> | 20.07 | 3.42 |
| Completeness [%] | 99.3 | 95.9 |
| Redundancy | 5.55 | 3.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
