1W31
YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE 5-HYDROXYLAEVULINIC ACID COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-02-16 |
Detector | ADSC CCD |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 102.570, 102.570, 168.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.280 - 1.900 |
R-factor | 0.1895 * |
Rwork | 0.190 |
R-free | 0.24930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ylv |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CCP4 |
Refinement software | RESTRAIN |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.058 | 0.419 |
Number of reflections | 1067852 | |
<I/σ(I)> | 8 | 1.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.9 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | ENZYME CONCENTRATION 1 MG/ML, PH 7.0 - 8.5, BUFFER 0.2 M TRIS-HCL, PRECIPITANT PEG 6000 (<10%), 70 MICROMOLAR ZINC SULPHATE, 6 MM BETA-MERCAPTOETHANOL, HANGING DROPS AS FOR PDB ENTRY 1AW5 WITH 10 MM 5-HYDROXYLAEVULINIC ACID IN DROP. |