1W2V
The 3-dimensional structure of a thermostable mutant of a xylanase (Xyn10A) from Cellvibrio japonicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 120 |
| Collection date | 2002-01-20 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 95.609, 95.609, 150.315 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.550 |
| R-factor | 0.151 |
| Rwork | 0.149 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1clx |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.452 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.080 | 0.290 |
| Number of reflections | 100882 | |
| <I/σ(I)> | 31.96 | 10.07 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.6 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
