1W2T
beta-fructosidase from Thermotoga maritima in complex with raffinose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 294 |
| Detector technology | CCD |
| Collection date | 2004-02-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.500, 114.700, 130.000 |
| Unit cell angles | 90.00, 98.90, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.870 |
| R-factor | 0.199 |
| Rwork | 0.198 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uyp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.103 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 129.099 | 30.000 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.062 | 0.184 |
| Number of reflections | 225700 | |
| <I/σ(I)> | 6 | 3.9 |
| Completeness [%] | 97.7 | 97.8 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | HANGING DROP TECHNIQUE 10% PEG 1000, 50 MM LI2SO4, 100 MM CITRATE PHOSPHATE BUFFER PH 4.2, CONCENTRATION PROTEIN 8 MG/ML, INCUBATION TEMPERATURE 20 C |
