1W2M
Ca-substituted form of E. coli aminopeptidase P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 H |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-17 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 208.359, 312.688, 160.198 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 182.570 - 2.400 |
| R-factor | 0.176 |
| Rwork | 0.175 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m35 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.254 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.040 | 0.160 |
| Number of reflections | 192310 | |
| <I/σ(I)> | 19.6 | 4.5 |
| Completeness [%] | 94.5 | 86 |
| Redundancy | 2.1 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | 25% PEG 4K, 0.1 MM EDTA, 0.1 M TRIS (PH 8.2), 0.2 M NA ACETATE CRYSTALS SOAKED IN 30% ISOPROPANOL PLUS 5 MM CACL2 FOR 10 MIN PRIOR TO FREEZING |
