1W24

Crystal Structure Of human Vps29

Experimental procedure

Experimental method	MAD
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-06-18
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	53.871, 53.871, 124.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 * - 2.100
R-factor	0.219
Rwork	0.218
R-free	0.24400
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.180 *
Data reduction software	AUTOMAR
Data scaling software	MARSCALE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.070	0.354 *
Total number of observations	54013 *
Number of reflections	10686 *
<I/σ(I)>	4.6	1.5
Completeness [%]	96.5	95.5
Redundancy	4.97 *	5.05

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		25%PEG4000, 10%ETHYENE GLYCOL, 100MM HEPES PH7.5, pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	25 (%)
2	1	reservoir	ethylene glycol	10 (%)
3	1	reservoir	HEPES	100 (mM)	pH7.5
4	1	drop	protein	3 (mg/ml)
5	1	drop		50 (mM)
6	1	drop	Tris-HCl	5 (mM)	pH7.5