1W1U
Inactive Urocanase-SA cocrystallized with urocanate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.429, 71.618, 129.158 |
| Unit cell angles | 90.00, 98.75, 90.00 |
Refinement procedure
| Resolution | 19.180 - 2.230 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uwk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.260 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.120 | 0.300 |
| Number of reflections | 1453010 | |
| <I/σ(I)> | 10 | 5 |
| Completeness [%] | 98.0 | 91 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 24% PEG8000, 0.1M SODIUM CACODYLATE (PH 5.8), 0.16M SODIUM ACETATE, 0.1% BETA-MERCAPTOETHANOL |
