1W1O
Native Cytokinin Dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Collection date | 2003-10-04 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 171.693, 171.693, 54.322 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.700 |
| R-factor | 0.199 |
| Rwork | 0.198 |
| R-free | 0.21500 |
| Structure solution method | SIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.933 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.080 | 0.340 |
| Number of reflections | 88469 | |
| <I/σ(I)> | 14.6 | 4.4 |
| Completeness [%] | 98.8 | 97.9 |
| Redundancy | 4.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | pH 4.60 |
