1W1D
Crystal Structure of the PDK1 Pleckstrin Homology (PH) domain bound to Inositol (1,3,4,5)-tetrakisphosphate
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-05 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.861,1.040 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.399, 58.922, 36.578 |
| Unit cell angles | 90.00, 101.48, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.500 |
| R-factor | 0.147 |
| Rwork | 0.145 |
| R-free | 0.20000 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.785 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | RSPS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.440 |
| Number of reflections | 23341 | |
| <I/σ(I)> | 17.7 | 3.2 |
| Completeness [%] | 98.6 | 99.5 |
| Redundancy | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.08 M MAGNESIUM ACETATE, 0.05M SODIUM CACODYLATE PH 6.5, 30 % PEG 4000 |
