1W0J
Beryllium fluoride inhibited bovine F1-ATPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 283.520, 107.374, 137.936 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB CODE 1E1Q NATIVE BOVINE MITOCHONDRIAL F1- ATPASE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.341 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.200 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | 0.354 |
| Number of reflections | 185113 | |
| <I/σ(I)> | 8.1 | 1.7 |
| Completeness [%] | 87.1 | 51.1 |
| Redundancy | 2 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | CRYSTALS WERE GROWN IN THE PRESENCE OF AZIDE, A KNOWN INHIBITOR, BUT THIS HAS NOT BEEN LOCATED IN THE STRUCTURE., pH 8.20 |
