1W08
STRUCTURE OF T70N HUMAN LYSOZYME
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-11-15 |
Detector | MSC |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.117, 56.178, 62.488 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.890 - 2.500 |
R-factor | 0.18487 |
Rwork | 0.177 |
R-free | 0.26759 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jsf |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.075 | 0.146 |
Number of reflections | 3903 | |
<I/σ(I)> | 14.2 | 6.5 |
Completeness [%] | 95.1 | 87.6 |
Redundancy | 6.4 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | HANGING DROP METHOD AT 293K. DROPLET RESERVOIR SOLUTION MIXED 1:1 WITH 10 MG/ML PROTEIN, 10 MM HEPES BUFFER PH 7.5, 0.4 M LICL. RESERVOIR 2.5 M NACL, 20 MM NAOAC PH 4.5. |