1VZM
OSTEOCALCIN FROM FISH ARGYROSOMUS REGIUS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-15 |
Detector | ADSC CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 48.700, 48.700, 119.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.750 - 1.400 |
R-factor | 0.1916 |
R-free | 0.23460 |
Structure solution method | SIRAS |
RMSD bond length | 0.011 |
RMSD bond angle | 0.030 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXCD |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.750 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.056 | 0.245 |
Number of reflections | 32430 | |
<I/σ(I)> | 34.8 | 5.2 |
Completeness [%] | 98.5 | 96.1 |
Redundancy | 22.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 294 | DROPS OF 1.5 MICRO-L OF PROTEIN SOLUTION, 10 MG/ML OSTEOCALCIN, 5 MM CACL2 AND 30 MM TRIS-HCL PH = 8, PLUS 1.5 MICRO-L OF WELL SOLUTION, MGCL2 0.2M, 30% PEG 4K AND TRIS-HCL 0.1M PH = 8.5, WERE EQUILIBRATED AGAINST 500 ML OF WELL SOLUTION AT 21DEGREES C OVER 2-3 WEEKS. |