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L. CASEI THYMIDYLATE SYNTHASE MUTANT E60Q BINARY COMPLEX WITH DUMP
Experimental procedure
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 78.300, 78.300, 242.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 26.010 |
| Data reduction software | PROCESS |
| Data scaling software | PROCESS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 * |
| High resolution limit [Å] | 2.500 * |
| Rmerge | 0.108 |
| Number of reflections | 11787 |
| Completeness [%] | 71.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | potassium phosphate | 20 (mM) | |
| 2 | 1 | drop | enzyme | 10 (mg/ml) | |
| 3 | 1 | drop | ammonium sulfate | 15-20 (mM) | |
| 4 | 1 | drop | dUMP | 5 (mM) | |
| 5 | 1 | drop | potassium phosphate | 20 (mM) |
