1VZ6

Ornithine Acetyltransferase (ORF6 Gene Product - Clavulanic Acid Biosynthesis) from Streptomyces clavuligerus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-15
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	63.663, 94.444, 180.377
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.080 - 2.750
R-factor	0.212
Rwork	0.212
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.110	2.900
High resolution limit [Å]	2.750	2.750
Rmerge	0.114	0.335
Number of reflections	29114
<I/σ(I)>	5.5	2.2
Completeness [%]	99.9	100
Redundancy	7	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		PROTEIN AT 12MG/ML MIXED 1:1 WITH A SOLUTION OF: 1.2M (NH4)2SO4, 40MM NH4H2PO4, 0.1M TRIS PH 8.0, 6% GLYCEROL.