1VZ2

PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN, Y73C/V427C/C255T MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-10
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	70.900, 99.600, 110.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.000 - 2.200
R-factor	0.165
Rwork	0.163
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h2w
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.081	0.185
Number of reflections	39994
<I/σ(I)>	16.4	4.4
Completeness [%]	99.3	95.1
Redundancy	3.8	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		SEE REMARK 1 REFERENCE 6, pH 8.50