1VZ0
Chromosome segregation protein Spo0J from Thermus thermophilus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-15 |
Detector | ADSC CCD |
Wavelength(s) | 0.9393, 0.9791, 0.9793 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 182.240, 295.150, 73.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.720 - 2.300 |
R-factor | 0.235 |
Rwork | 0.235 |
R-free | 0.27800 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.290 |
Rmerge | 0.078 | 0.311 |
Number of reflections | 87653 | |
<I/σ(I)> | 12.1 | 3 |
Completeness [%] | 98.3 | 90.6 |
Redundancy | 3.5 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | pH 4.50 |