1VYW

Structure of CDK2/Cyclin A with PNU-292137

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-20
Detector	ADSC CCD
Spacegroup name	P 62 2 2
格子定数 [Å]	184.223, 184.223, 214.720
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	29.860 - 2.300
Rwork	0.229
R-free	0.25800
Structure solution method	OTHER
結合長の平均二乗偏差(RMSD) [Å]	0.007
結合角の平均二乗偏差(RMSD) [度]	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNX
Refinement software	CNX (2002)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	35.000	2.380
分解能 [Å] (高)	2.300	2.300
Rmerge_l_obs	0.081	0.690
独立反射数	94577
<I/σ(I)>	16	2.3
完全性 [%]	99.0	97
冗長性	4.8	4.2

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1		7		20% AMMONIUM SULPHATE, 1M KCL, 40MM HEPES PH 7