1VYV
beta4 subunit of Ca2+ channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Collection date | 2004-02-15 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.300, 83.200, 72.300 |
| Unit cell angles | 90.00, 94.90, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vyu |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | GCOMO |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.072 | 0.282 |
| Number of reflections | 47712 | |
| Completeness [%] | 99.0 | 98.5 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 20 MM NA-HEPES PH 7.2, 250 MM NACL AND 5% PEG3350 |
