1VYH
PAF-AH Holoenzyme: Lis1/Alfa2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 205.770, 101.220, 246.130 |
| Unit cell angles | 90.00, 98.06, 90.00 |
Refinement procedure
| Resolution | 200.000 - 3.400 |
| R-factor | 0.265 |
| Rwork | 0.264 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fxw |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.520 |
| High resolution limit [Å] | 3.391 | 3.390 |
| Rmerge | 0.096 | 0.320 |
| Number of reflections | 139752 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 96.4 | 96.4 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 16% PEG8K 0.2M KSCN, pH 8.50 |
