1VYH
PAF-AH Holoenzyme: Lis1/Alfa2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-21 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 205.770, 101.220, 246.130 |
Unit cell angles | 90.00, 98.06, 90.00 |
Refinement procedure
Resolution | 200.000 - 3.400 |
R-factor | 0.265 |
Rwork | 0.264 |
R-free | 0.30700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fxw |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.520 |
High resolution limit [Å] | 3.391 | 3.390 |
Rmerge | 0.096 | 0.320 |
Number of reflections | 139752 | |
<I/σ(I)> | 2 | |
Completeness [%] | 96.4 | 96.4 |
Redundancy | 1.8 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 16% PEG8K 0.2M KSCN, pH 8.50 |