1VYG

schistosoma mansoni fatty acid binding protein in complex with arachidonic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	42.290, 91.010, 35.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.217
Rwork	0.213
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDBE ENTRY 1VYF
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.067	0.170
Number of reflections	5746
<I/σ(I)>	19.6	8.8
Completeness [%]	95.5	86.9
Redundancy	5.5	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		30% PEG 8K,0.1M MES, 0.2M NA ACETATE, 5MM BME, pH 6.00