1VYD

Crystal structure of cytochrome C2 mutant G95E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	NONIUS
Temperature [K]	294
Detector technology	IMAGE PLATE
Detector	MAC Science DIP-2030
Spacegroup name	H 3 2
Unit cell lengths	100.230, 100.230, 162.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.300
Rwork	0.198
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	WILD TYPE CYTOCHROME C2
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.300
Rmerge	0.087
Number of reflections	12459
<I/σ(I)>	13.27
Completeness [%]	88.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		pH 7.50