1VWN

STREPTAVIDIN-CYCLO-AC-[CHPQFC]-NH2, PH 4.8

Experimental procedure

Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	I 41 2 2
Unit cell lengths	57.690, 57.690, 175.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 1.850
R-factor	0.205
Rwork	0.205
R-free	0.25000
RMSD bond length	0.019
RMSD bond angle	25.100 *
Data reduction software	bioteX ((MSC))
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.320 *
Rmerge	0.093
Total number of observations	61076 *
Number of reflections	20636
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5 *	20 *	Pahler, A., (1987) J. Biol. Chem., 262, 13933. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	drop	potassium acetate	50 (mM)
3	1	drop		200 (mM)	pH4.5
4	1	reservoir	ammonium sulfate	30 (%sat)
5	1	reservoir	potassium acetate	50 (mM)
6	1	reservoir		200 (mM)