1VWH

STREPTAVIDIN COMPLEXED WITH THE HEAD-TO-TAIL DISULFIDE-BONDED PEPTIDE DIMER OF CYCLO-AC-[CHPQGPPC]-NH2, PH 3.5

Experimental procedure

Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	I 41 2 2
Unit cell lengths	58.180, 58.180, 174.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 1.480
R-factor	0.198
Rwork	0.198
R-free	0.23900
RMSD bond length	0.019
RMSD bond angle	26.200 *
Data reduction software	bioteX ((MSC))
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.330 *
Rmerge	0.059
Total number of observations	45610 *
Number of reflections	26527
Redundancy	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5 *	20 *	Pahler, A., (1987) J. Biol. Chem., 262, 13933. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	drop	potassium acetate	50 (mM)
3	1	drop		200 (mM)	pH4.5
4	1	reservoir	ammonium sulfate	30 (%sat)
5	1	reservoir	potassium acetate	50 (mM)
6	1	reservoir		200 (mM)