1VTO
1.9 A RESOLUTION REFINED STRUCTURE OF TBP RECOGNIZING THE MINOR GROOVE OF TATAAAAG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Detector technology | IMAGE PLATE |
| Detector | FUJI |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.000, 147.000, 57.000 |
| Unit cell angles | 90.00, 90.50, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.900 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.26400 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.570 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.880 |
| Number of reflections | 51793 |
| Completeness [%] | 93.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.2 | 277 | pH 6.20, VAPOR DIFFUSION, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | 2 | 3 |
| 10 | 1 | 2 | MES | 29 | 30 |
| 2 | 1 | 1 | DTT | 5 | 6 |
| 3 | 1 | 1 | GLYCEROL | 8 | 9 |
| 4 | 1 | 1 | MES | 11 | 12 |
| 5 | 1 | 1 | KCL | 14 | 15 |
| 6 | 1 | 1 | NH4 ACETATE | 17 | 18 |
| 7 | 1 | 2 | WATER | 20 | 21 |
| 8 | 1 | 2 | DTT | 23 | 24 |
| 9 | 1 | 2 | GLYCEROL | 26 | 27 |
