1VTE
MOLECULAR STRUCTURE OF NICKED DNA. MODEL A4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 298 |
| Detector technology | DIFFRACTOMETER |
| Detector | RIGAKU AFC-5R |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.990, 44.030, 66.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 3.000 |
| R-factor | 0.182 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Phasing software | ULTIMA |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 553 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 277 | pH 5.00, VAPOR DIFFUSION, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | 2 | 3 |
| 2 | 1 | 1 | MPD | 5 | 6 |
| 3 | 1 | 1 | MGCL2 | 8 | 9 |
| 4 | 1 | 1 | NA CACODYLATE | 11 | 12 |
| 5 | 1 | 1 | SPERMINE_HCL | 14 | 15 |
| 6 | 1 | 2 | WATER | 17 | 18 |
| 7 | 1 | 2 | MPD | 20 | 21 |
