1VTD

UNUSUAL HELICAL PACKING IN CRYSTALS OF DNA BEARING A MUTATION HOT SPOT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	273
Detector technology	DIFFRACTOMETER
Detector	NICOLET
Spacegroup name	H 3
Unit cell lengths	65.900, 65.900, 47.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.800
R-factor	0.153
Refinement software	NUCLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.800
Number of reflections	1584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.1	277	pH 6.10, VAPOR DIFFUSION, temperature 277.00K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	WATER	2	3
2	1	1	CACODYLATE	5	6
3	1	1	SPERMINE	8	9
4	1	1	MG ACETATE	11	12
5	1	2	WATER	14	15
6	1	2	MPD	17	18