1VST
Symmetric Sulfolobus solfataricus uracil phosphoribosyltransferase with bound PRPP and GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2005-02-22 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.954 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 122.200, 122.200, 62.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.800 |
Rwork | 0.226 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xtv |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.152 | 0.418 |
Number of reflections | 7102 | |
<I/σ(I)> | 15.9 | 4.2 |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 10% PEG8000, 0.1M sodium-cacodylate, pH6.5, 200mM magnesium-chloride, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |