1VSM

ASV INTEGRASE CORE DOMAIN IN CITRATE BUFFER PH 5.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1998-01-09
Detector	MACSCIENCE
Spacegroup name	P 43 21 2
Unit cell lengths	67.091, 67.091, 79.237
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.150
R-factor	0.146
Rwork	0.146
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1asv
RMSD bond length	0.013
RMSD bond angle	24.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.190
High resolution limit [Å]	2.130	2.130
Rmerge	0.080 *
Total number of observations	28268 *
Number of reflections	9166
<I/σ(I)>	3	10
Completeness [%]	85.7	86.5
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		Bujacz, G., (1995) J. Mol. Biol., 253, 333. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6-7.5 (mg/ml)
2	1	reservoir	HEPES	100 (mM)
3	1	reservoir	isopropanol	10 (%)
4	1	reservoir	PEG4000	20 (%)