1VSH

ASV INTEGRASE CORE DOMAIN WITH ZN(II) COFACTORS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-07-22
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	66.080, 66.080, 80.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.950
R-factor	0.173 *
Rwork	0.173
Structure solution method	ISOSTRUCTURAL TO PDB ENTRY 1VSF
RMSD bond length	0.013
RMSD bond angle	0.043
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.980
High resolution limit [Å]	1.950	1.950
Number of reflections	13238
<I/σ(I)>	13.4	2.6
Completeness [%]	97.0	94.7
Redundancy	3.7	3.15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5		PROTEIN WAS CRYSTALLIZED FROM 20% PEG 4000, 10% ISOPROPANOL, 100 MM HEPES, PH 7.5, THEN SOAKED IN 100 MM ZNCL2.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	20 (%(w/v))
2	1	reservoir	HEPES	100 (mM)
3	1	reservoir	isopropanol	10 (%)
4	1	drop	protein	6-7.5 (mg/ml)