1VSG

2.9 ANGSTROMS RESOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN OF A VARIANT SURFACE GLYCOPROTEIN FROM TRYPANOSOMA BRUCEI

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	95.500, 97.500, 123.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 * - 2.900
R-factor	0.22 *
Rwork	0.220
RMSD bond length	0.015
RMSD bond angle	2.800
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.900 *
Rmerge	0.087 *
Total number of observations	56659 *
Number of reflections	24767 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop		0.01 (%(w/v))
4	1	reservoir		58 (%sat)
5	1	reservoir	Tris-HCl	20 (mM)
6	1	reservoir		0.01 (%)