1VSF

ASV INTEGRASE CORE DOMAIN WITH MN(II) COFACTOR AND HEPES LIGAND, HIGH MG CONCENTRATION FORM

Experimental procedure

Source type	ROTATING ANODE
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1994-10-20
Detector	MAR scanner 300 mm plate
Spacegroup name	P 43 21 2
Unit cell lengths	66.240, 66.240, 81.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.050
R-factor	0.13
R-free	0.18900
RMSD bond length	0.013
RMSD bond angle	0.047
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	2.050 *
Rmerge	0.085
Number of reflections	11953
Completeness [%]	99.9
Redundancy	5.53

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		pH 7.5 THE PROTEIN WAS CRYSTALLIZED FROM 20% PEG 4000, 10% ISOPROPANOL, 100 MILLIMOLAR HEPES PH 7.5. CRYSTALS WERE THEN SOAKED IN 10 MILLIMOLAR MNCL2.