1VRT

HIGH RESOLUTION STRUCTURES OF HIV-1 RT FROM FOUR RT-INHIBITOR COMPLEXES

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Detector technology	IMAGE PLATE
Collection date	1994-03-03
Detector	FUJI
Spacegroup name	P 21 21 21
Unit cell lengths	141.800, 116.700, 66.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.200
R-factor	0.186
Rwork	0.186
RMSD bond length	0.015
RMSD bond angle	25.000 *
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.200	2.200 *
Rmerge	0.129
Total number of observations	245640 *
Number of reflections	49373	4732 *
Completeness [%]	86.8	67.8 *
Redundancy	4.97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3400	6 (%)
2	1	reservoir	citrate/phosphate