1VRM

Crystal structure of the apbe protein (tm1553) from thermotoga maritima msb8 at 1.58 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-22
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97950,1.00003
Spacegroup name	P 21 21 21
Unit cell lengths	57.742, 76.946, 86.587
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.610 - 1.580
Rwork	0.157
R-free	0.19100
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.564
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.610	20.610	1.620
High resolution limit [Å]	1.500	7.070	1.580
Rmerge	0.096	0.050	0.703
Number of reflections	51395
<I/σ(I)>	5	11.5	0.9
Completeness [%]	96.2
Redundancy	3.2	3.1	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	5	277	10.0% MPD, 0.1M Citrate pH 5.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K