1VRA

Crystal structure of Arginine biosynthesis bifunctional protein argJ (10175521) from Bacillus halodurans at 2.00 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-21
Detector	ADSC
Spacegroup name	P 41 21 2
Unit cell lengths	70.658, 70.658, 222.292
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.810 - 2.000
R-factor	0.14056
Rwork	0.140
R-free	0.15782
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.426
Data reduction software	MOSFLM
Data scaling software	SCALA (5.0))
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.810	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.065	0.109
Number of reflections	38627
<I/σ(I)>	21	10.2
Completeness [%]	98.8	90.7
Redundancy	5.3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	4.2	277	40.0% Ethylene-Glycol, 0.2M (NH4)2SO4, 0.1M Phosphate Citrate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	4.2	277	40.0% Ethylene-Glycol, 0.2M (NH4)2SO4, 0.1M Phosphate Citrate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K