1VR8
Crystal structure of GTP binding regulator (TM1622) from Thermotoga Maritima at 1.75 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.99186, 0.97951, 0.97933 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 57.730, 57.730, 87.944 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.310 - 1.750 |
| R-factor | 0.15019 |
| Rwork | 0.149 |
| R-free | 0.18179 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.490 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (5.0) |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.310 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 16407 | |
| <I/σ(I)> | 14.4 | 2.3 |
| Completeness [%] | 93.2 | 63.9 |
| Redundancy | 4.4 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 277 | 12.0% PEG-20000, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |
