1VR6
Crystal structure of Phospho-2-dehydro-3-deoxyheptonate aldolase (DAHP synthase) (TM0343) from Thermotoga Maritima at 1.92 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-03 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.151, 74.226, 249.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.760 - 1.920 |
R-factor | 0.1732 |
Rwork | 0.171 |
R-free | 0.21519 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rzm |
RMSD bond length | 0.016 |
RMSD bond angle | 1.516 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.760 | 1.960 |
High resolution limit [Å] | 1.920 | 1.930 |
Number of reflections | 102464 | |
<I/σ(I)> | 23.07 | 1.49 |
Completeness [%] | 98.8 | 90.38 |
Redundancy | 3.57 | 2.63 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 5.5 | 293 | 35.0% Ethylene-Glycol, 0.1M Acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 293K |