1VR2
HUMAN VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR 2 (KDR) KINASE DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.410, 96.040, 38.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.400 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fgk |
| RMSD bond length | 0.012 |
| RMSD bond angle | 22.860 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.052 * | 0.196 * |
| Number of reflections | 17234 | |
| <I/σ(I)> | 23.6 | 3.4 |
| Completeness [%] | 93.0 | 81.5 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 | 4 * | drop consists of equal volume of protein and mother-liquor * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | mother-liquor |
| 3 | 1 | reservoir | ammonium sulfate | 2 (M) | mother-liquor |
| 4 | 1 | reservoir | mPEG550 | 2 (%(v/v)) | mother-liquor |
| 5 | 1 | reservoir | beta-mercaptoethanol | 50 (mM) |
