1VR2

HUMAN VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR 2 (KDR) KINASE DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	95.410, 96.040, 38.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.215
Rwork	0.215
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fgk
RMSD bond length	0.012
RMSD bond angle	22.860 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.052 *	0.196 *
Number of reflections	17234
<I/σ(I)>	23.6	3.4
Completeness [%]	93.0	81.5
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2	4 *	drop consists of equal volume of protein and mother-liquor *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	HEPES	100 (mM)	mother-liquor
3	1	reservoir	ammonium sulfate	2 (M)	mother-liquor
4	1	reservoir	mPEG550	2 (%(v/v))	mother-liquor
5	1	reservoir	beta-mercaptoethanol	50 (mM)