1VQZ

Crystal structure of a putative lipoate-protein ligase a (sp_1160) from streptococcus pneumoniae tigr4 at 1.99 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-10
Detector	ADSC
Wavelength(s)	0.979724,0.956885,0.979691
Spacegroup name	P 21 21 21
Unit cell lengths	45.503, 70.280, 113.629
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.100 - 1.990
R-factor	0.16211
Rwork	0.159
R-free	0.21691
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.530
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.100	2.100
High resolution limit [Å]	1.990	1.990
Number of reflections	25714
<I/σ(I)>	14.2	3
Completeness [%]	99.8	98.8
Redundancy	10.4	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	6.8	277	0.2M Na2NO3, 20.0% PEG-3350, No Buffer pH 6.8, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K