1VQW
Crystal structure of a protein with similarity to flavin-containing monooxygenases and to mammalian dimethylalanine monooxygenases
Replaces: 1YBPExperimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920, 0.97940, 0.95000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.590, 72.640, 80.350 |
| Unit cell angles | 99.00, 107.09, 102.02 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.23900 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.409 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE (& SHARP) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 47252 | |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 98.4 | 96.2 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 298 | PEG 4000, Ammonium acetate, Sodium citrate, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K, pH 5.80 |
