1VQW
Crystal structure of a protein with similarity to flavin-containing monooxygenases and to mammalian dimethylalanine monooxygenases
Replaces: 1YBPExperimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97920, 0.97940, 0.95000 |
Spacegroup name | P 1 |
Unit cell lengths | 59.590, 72.640, 80.350 |
Unit cell angles | 99.00, 107.09, 102.02 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.23900 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.409 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE (& SHARP) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 47252 | |
<I/σ(I)> | 8.2 | |
Completeness [%] | 98.4 | 96.2 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 298 | PEG 4000, Ammonium acetate, Sodium citrate, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K, pH 5.80 |