1VQU

Crystal structure of Anthranilate phosphoribosyltransferase 2 (17130499) from Nostoc sp. at 1.85 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-19
Detector	ADSC
Spacegroup name	P 1 21 1
Unit cell lengths	67.556, 47.233, 99.376
Unit cell angles	90.00, 92.72, 90.00

Refinement procedure

Resolution	99.050 - 1.850
R-factor	0.18131
Rwork	0.179
R-free	0.22060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kgz
RMSD bond length	0.017
RMSD bond angle	1.569
Data reduction software	MOSFLM
Data scaling software	SCALA (4.2))
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.050	1.900
High resolution limit [Å]	1.850	1.850
Number of reflections	52559
<I/σ(I)>	12.9	1.3
Completeness [%]	98.4	99.7
Redundancy	3.3	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		293	20.025% PEG 3350, 0.2M Acetate_Na, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K