1VQP
The structure of the transition state analogue "RAP" bound to the large ribosomal subunit of haloarcula marismortui
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Detector technology | CCD |
| Collection date | 2004-04-24 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 211.610, 298.189, 574.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.250 |
| Rwork | 0.218 |
| R-free | 0.24690 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1s72 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.058 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.310 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 841236 | |
| <I/σ(I)> | 22.1 | 1 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
