1VQJ

GENE V PROTEIN MUTANT WITH VAL 35 REPLACED BY ILE 35 (V35I)

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-05
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	76.140, 28.110, 42.560
Unit cell angles	90.00, 103.42, 90.00

Refinement procedure

Resolution	5.000 - 1.800
R-factor	0.206
Rwork	0.206
RMSD bond length	0.013
RMSD bond angle	2.520
Data reduction software	MAR (600)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.047
Total number of observations	26381 *
Number of reflections	8055
Completeness [%]	97.9
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.5 (mM)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop	PEG3400	15 (%(w/v))
4	1	reservoir	PEG3400	12 (%)