1VQD
GENE V PROTEIN MUTANT WITH VAL 35 REPLACED BY ILE 35 AND ILE 47 REPLACED BY LEU 47 (V35I, I47L)
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1994-05 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 76.120, 28.140, 42.520 |
Unit cell angles | 90.00, 103.48, 90.00 |
Refinement procedure
Resolution | 5.000 - 1.820 |
R-factor | 0.21 |
Rwork | 0.210 |
RMSD bond length | 0.013 |
RMSD bond angle | 2.560 |
Data reduction software | MAR (600) |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.820 * |
Rmerge | 0.041 |
Number of reflections | 7371 |
Completeness [%] | 91.1 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.6 * | Skinner, M.M., (1994) Proc.Nat.Acad.Sci.USA, 91, 2071. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.5 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 0.025 (M) | |
3 | 1 | drop | PEG4000 | 10 (%) | |
4 | 1 | reservoir | PEG4000 | 10 (%) |