1VQA
GENE V PROTEIN MUTANT WITH VAL 35 REPLACED BY ALA 35 AND ILE 47 REPLACED BY LEU 47 (V35A, I47L)
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-05 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.000, 28.030, 42.630 |
| Unit cell angles | 90.00, 103.53, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.800 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.570 |
| Data reduction software | MAR (600) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 * |
| Rmerge | 0.038 |
| Number of reflections | 8003 |
| Completeness [%] | 97.3 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.6 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.025 (M) | |
| 3 | 1 | drop | PEG4000 | 10 (%) | |
| 4 | 1 | reservoir | PEG4000 | 10 (%) |
