1VQ4
The structure of the transition state analogue "DAA" bound to the large ribosomal subunit of Haloarcula marismortui
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Detector technology | CCD |
Collection date | 2004-08-03 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 212.998, 301.034, 575.272 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.700 |
R-factor | 0.2065 |
Rwork | 0.190 |
R-free | 0.23020 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1s72 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.050 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.790 |
High resolution limit [Å] | 2.690 | 2.690 |
Number of reflections | 506033 | |
<I/σ(I)> | 23.5 | 2 |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |