1VQ3
Crystal structure of Phosphoribosylformylglycinamidine synthase, purS subunit (EC 6.3.5.3) (TM1244) from Thermotoga maritima at 1.90 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-13 |
| Detector | ADSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.270, 73.433, 92.567 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.310 - 1.900 |
| R-factor | 0.1799 |
| Rwork | 0.178 |
| R-free | 0.23450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t4a |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.553 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (4.2)) |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.310 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 29272 | |
| <I/σ(I)> | 16.3 | 2.3 |
| Completeness [%] | 91.9 | 62.7 |
| Redundancy | 3.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 277 | 20.0% Glycerol, 24.0% PEG-1500,, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
