1VQ1
Crystal structure of N5-glutamine methyltransferase, HemK(EC 2.1.1.-) (TM0488) from Thermotoga maritima at 2.80 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-13 |
Detector | ADSC |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 140.973, 58.611, 85.667 |
Unit cell angles | 90.00, 109.46, 90.00 |
Refinement procedure
Resolution | 36.090 - 2.800 |
R-factor | 0.21156 |
Rwork | 0.208 |
R-free | 0.27281 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nv8 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.507 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (4.2)) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.090 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 15828 | |
<I/σ(I)> | 13.3 | 1.7 |
Completeness [%] | 96.0 | 73.3 |
Redundancy | 3.4 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7.5 | 293 | 5.0% MPD, 10.0% PEG-6000, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |